Background: The most sensitive and widely used liver enzymes for detecting are the aminotransferases. The purpose of this study was to evaluate the effects of diving effect of male scuba divers in different depth on aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Materials and Methods: 12 members of the Red Crescent and rescue divers recruited voluntarily from Kohgiluyeh and Boyer-Ahmad Province , with an average age of 28.83 ± 2.63 years, weight 77.5 ± 7.16 kg ,and height 175.33 ± 2.88 cm participated in this study.To perform this research project, a pre-test and post-test group with repeated measures was used to test the first day of the depth of 10 m, the second day in a depth of 20 meters and the third day in a depth of 30 meters for 20 minutes by the 40 to 50% heart rate reserve as they sweep diving. Participants at a height of three meters to the water level had safety stop for five minutes. Before and after diving seven cc bloods were taken from all subjects. In order to analysis of data mean and standard deviation were used and inferential statistics analysis of variance with repeated measures was used to evaluate the normal distribution. Results: Increase environment pressure caused by Scuba deep Diving had no significant effect on serum AST (p > 0.05). Also, increase environment pressure was significant impact on serum ALT from Scuba Diving (p < 0.05) so that, serum ALT in the depths of 20 and 30 meters was significantly reduced compared to the depth of 10 meters. Also diving regardless of pressure (depth) no significant effect on serum ALT (p > 0.05). Conclusion: It seems that the alter in depth in the range of 10 to 30 meters does not cause significant changes in serum levels of these two liver enzymes.