نشریه علمی پژوهشی طب انتظامی Journal of Police Medicine
Volume 7, Issue 4 (2018)
J Police Med 2018, 7(4) |
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jalili S, shirzad H. Comparison of different DNA extraction and preparation methods for forensic application of Blood Samples. J Police Med 2018; 7 (4)
URL: http://jpmed.ir/article-1-713-en.html
URL: http://jpmed.ir/article-1-713-en.html
1- NAJA Institute of Law Enforcement and Social Studies - Institute of Technology Management , jalili.shirin@yahoo.com
2- NAJA Institute of Law Enforcement and Social Studies - Institute of Technology Management
2- NAJA Institute of Law Enforcement and Social Studies - Institute of Technology Management
English Extended Abstract: (2851 Views)
Abstract
Introduction: Extraction of DNA from forensic samples is the first and one of the most crucial steps of any genetic forensic test. Hence, obtaining a suitable protocol of extraction DNA that is easy to extract, cost, time, and quality which is essential in the forensic genetic research.
Aims: The present study was carried out to achieve a suitable, faster and low cost method of DNA extraction from different sample for further molecular study.
Materials & Methods: After preparation of samples, three different DNA extraction methods, including chelex, non-enzymatic and modified rapid alkaline lysis were evaluated. In order to compare the above mentioned methods for evaluation ease of extraction, low cost and time , the quality and quantity of DNA obtained from each method considering OD, DNA concentration and PCR efficiency were investigated.
Results: The findings of this study show that despite the availability and lower cost and time of manual methods, these methods can be substitute for kit method.
Article Type: Original Research |
Subject:
Forensic Medicine
Received: 2018/06/19 | Accepted: 2018/09/17 | Published: 2018/10/7
Received: 2018/06/19 | Accepted: 2018/09/17 | Published: 2018/10/7
English Expanded Abstract [HTML 17 KB] (103 Download)
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