Volume 10, Issue 4 (10-2021)                   J Police Med 2021, 10(4): 225-240 | Back to browse issues page


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Mahdavi R, Hassani M, Roostaie A, Khalili M, Haghbin Nazar Pak M. Design and Manufacture of Modified Alginate Spray as a New Wound Dressing to Control Bleeding and Wound Healing. J Police Med. 2021; 10 (4) :225-240
URL: http://jpmed.ir/article-1-1035-en.html
1- Department of Biomedical Engineering, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
2- Department of Biomedical Engineering, Faculty of Biomedical Engineering, Science & Research Branch, Islamic Azad University, Tehran, Iran
3- Police Technology & Equipment Department, Police Sciences & Social Studies Institute, Tehran, Iran , ali.roostaie1@gmail.com
Abstract:   (285 Views)
Aims: Alginate is one of the substances that is considered as a promising candidate for wound healing. The aim of this study was to design and manufacture a dressing spray based on alginate / calcium chloride / gelatin alginate to control bleeding and wound healing.
Materials & Methods: This experimental research was conducted in the research laboratory of Amirkabir University from December 2019 to January 2020. In this study, saline phosphate buffer solution was prepared first. Then, their sterilization process was performed by autoclave and MTT salt solution was prepared during a process. In order to prepare an alginate-based spray dressing, we adjusted the concentration of the solution so that it could escape from the spray pore. On the other hand, in order to obtain gel alginate with suitable strength in equal proportions of calcium chloride concentrations, aqueous solution of gelatin was prepared for copolymerization with alginate. Finally, the bonds in the proposed dressing were evaluated by FTIR. Also, the degree of degradability, water absorption, changes in weight and pH, physical properties and microstructure of the extrinsic biological response were investigated in the designed dressing. Scanning electron microscopy and infrared spectroscopy were used to examine the dressing structure resulting from the ratio of different amounts of materials involved.
Findings: The results showed that the amount of water absorption in the sample and their degradation rate were AGC3> AGC4> AGC2> AGC1. In the study of biocompatibility, it was found that despite better results in AGC3, the degree of non-toxicity of AGC2 group was higher and also showed better cell adhesion behavior. Therefore, according to the results, AGC2 sample was selected as the final sample.
Conclusion: The results revealed that increasing calcium chloride levels could lead to increased alginate hydrogel formation. Also, the presence of gelatin and calcium chloride in the constant concentration of alginate will reduce the formation time of the spray hydrogel until they create space barriers.
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Article Type: Original Research | Subject: Police Medicine Related Technologies
Received: 2021/07/18 | Accepted: 2021/08/25 | Published: 2021/10/2

References
1. KC Dee, DA Puleo R. Bizios, an introduction to tissue-biomaterial interactions, Cell Mol. Biol. 2004;419-25.
2. A Falabella, R Kirsner. Wound healing, Basic & clinical dermatology, Taylor and Francis, Boca Raton, Florida, 2005. [DOI:10.1201/b14164]
3. B. Jayesh. The history of wound care. Journal Am College Certified Wound Specialists. 2011;3:65-6. [DOI:10.1016/j.jcws.2012.04.002] [PMID] [PMCID]
4. M Simkhah, M Dehghan Esmatabadi, M Zeinoddini, N Pourmahdi. In Silico Design of a Hybrid Structure as Positive Control for Simultaneous Detection of 4 Pathogenic Agents by PCR Method. J Police Med. 2020;9(1):9-16
5. AS Halim, TL Khoo, SJM Yussof. Biologic and synthetic skin substitutes: an overview, Indian J Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India 2010;43(Suppl 23) [DOI:10.4103/0970-0358.70712] [PMID] [PMCID]
6. K Vowden, P Vowden. Wound dressings: principles and practice, surgery. Oxford. 2017;35(9):489-94. [DOI:10.1016/j.mpsur.2017.06.005]
7. J Kucińska-Lipka, I Gubanska, H Janik. Bacterial cellulose in the field of wound healing and regenerative medicine of skin: recent trends and future prospectives. Polymer Bulletin 2015;72(9):2399-419. [DOI:10.1007/s00289-015-1407-3]
8. G Winter. Formation of the scab and the rate of epithelisation of superficial wounds in the skin of the young domestic pig. 1962, J Wound Care 1995;4(8):366.
9. MG Dunn, CJ Doillon, RA Berg, RM Olson, FH Silver. Wound healing using a collagen matrix: effect of DC electrical stimulation. J Biomed Material Res. 1988;22(S13):191-206. [DOI:10.1002/jbm.820221310] [PMID]
10. J Klimentová, J Stulík. Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria. Microbiol Res. 2015;170:1-9. [DOI:10.1016/j.micres.2014.09.006] [PMID]
11. M Ishihara, K Nakanishi, K Ono, M Sato, M Kikuchi, Y Saito, et al. Uenoyama, photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials. 2002;23(3):833-40. [DOI:10.1016/S0142-9612(01)00189-2]
12. GD Mogoşanu, AM Grumezescu. Natural and synthetic polymers for wounds and burns dressing. Int J Pharmaceutic. 2014;463(2):127-36. [DOI:10.1016/j.ijpharm.2013.12.015] [PMID]
13. J Stana, Večslojne. Medicin matrice z modificiranim sproščanjem pentoksifilina za zdravljenje kronične venozne razjede, Univerza v Mariboru, Medicinska fakulteta, 2018.
14. O Catanzano, M Straccia, A Miro F, Ungaro, I Romano, G. Mazzarella, et al. Spray-by-spray in situ cross-linking alginate hydrogels delivering a tea tree oil microemulsion. Europ J Pharmaceutical Sci. 2015;66:20-8. [DOI:10.1016/j.ejps.2014.09.018] [PMID]
15. H Ueno, H Yamada, I Tanaka, N Kaba, M Matsuura, M Okumura, et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs, Biomaterials. 1999;20(15):1407-14. [DOI:10.1016/S0142-9612(99)00046-0]
16. A Sood, MS Granick, NL Tomaselli. Wound dressings and comparative effectiveness data, Advances in wound care. 2014;3(8):511-29. [DOI:10.1089/wound.2012.0401] [PMID] [PMCID]
17. A Sosnik. Alginate particles as platform for drug delivery by the oral route: state-of-the-art. Int Scholar Res Notic. 2014. [DOI:10.1155/2014/926157] [PMID] [PMCID]
18. S Mohammadi, SS Shafiei, M Asadi-Eydivand, M Ardeshir, M Solati-Hashjin. Graphene oxide-enriched poly(ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications. J Bioactive Compat Polymer. 2017;32(3):325-42. [DOI:10.1177/0883911516668666]
19. SI Jeong, MD Krebs, CA Bonino, JE Samorezov, SA Khan, E Alsberg. Electrospun chitosan-alginate nanofibers with in situ polyelectrolyte complexation for use as tissue engineering scaffolds. Tissue Eng Part. 2011;17(1-2):59-70. [DOI:10.1089/ten.tea.2010.0086] [PMID]
20. DL Pavia, GM Lampman, GS Kriz, JA Vyvyan. Introduction to spectroscopy. Nelson Education; 2014.
21. R Imani, S Emami, S Faghihi. Nano-graphene oxide carboxylation for efficient bioconjugation applications: a quantitative optimization approach. J Nanoparticle Res. 2015;17(2):1. [DOI:10.1007/s11051-015-2888-6]
22. E Jain, A Srivastava, A Kumar. Macroporous interpenetrating cryogel network of poly (acrylonitrile) and gelatin for biomedical applications. J Material Sci: Material Med. 2009;20(1):173-9. [DOI:10.1007/s10856-008-3504-4] [PMID]
23. A Blandino, M Macías. Formation of calcium alginate gel capsules: Influence of sodium alginate and CaCl2 concentration on gelation kinetics. J Biosci Bioeng. 1999;88(6):686-9. [DOI:10.1016/S1389-1723(00)87103-0]
24. TR Cuadros, AA Erices, JM Aguilera. Porous matrix of calcium alginate/gelatin with enhanced properties as scaffold for cell culture. J Mechanical Behav Biomed Material. 2015;46:331-42. [DOI:10.1016/j.jmbbm.2014.08.026] [PMID]
25. N Cao, X Chen, D Schreyer. Influence of calcium ions on cell survival and proliferation in the context of an alginate hydrogel. ISRN Chemical Eng. 2012 [DOI:10.5402/2012/516461]
26. CJ Knill, JF Kennedy, J Mistry, M Miraftab, G Smart, MR Groocock, et al. Alginate fibers modified with unhydrolysed and hydrolysed chitosans for wound dressings. Carbohydr Polym. 2004;55(1):65-76. [DOI:10.1016/j.carbpol.2003.08.004]
27. S Opasanon, P Muangman, N Namviriyachote. Clinical effectiveness of alginate silver dressing in outpatient management of partial-thickness burns. Int Wound J. 2010;7(6):467-71. [DOI:10.1111/j.1742-481X.2010.00718.x] [PMID] [PMCID]

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