Comparison of two immunogenic recombinant constructs based on T cell epitopes (extracted from whole proteome screening of Salmonella typhi bacteria) against typhoid fever; an immunoinformatic study

beiranvand, Fateme; Shams, Nemat; Jaydari, Amin; Nazifi, Narges; Khademi, Peyman

doi:10.30505/13.1.11

نشریه علمی پژوهشی طب انتظامی Journal of Police Medicine

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‎ 10.30505/13.1.11

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beiranvand F, Shams N, Jaydari A, Nazifi N, Khademi P. Comparison of two immunogenic recombinant constructs based on T cell epitopes (extracted from whole proteome screening of Salmonella typhi bacteria) against typhoid fever; an immunoinformatic study. J Police Med 2024; 13 (1) : e11
URL: http://jpmed.ir/article-1-1276-en.html

Comparison of two immunogenic recombinant constructs based on T cell epitopes (extracted from whole proteome screening of Salmonella typhi bacteria) against typhoid fever; an immunoinformatic study

Fateme Beiranvand¹, Nemat Shams ^*², Amin Jaydari³, Narges Nazifi⁴, Peyman Khademi¹

1- Department of Microbiology and food hygiene, Faculty of Veterinary Medicine, Lorestan University, Tehran, Iran
2- Department of Microbiology and food hygiene, Faculty of Veterinary Medicine, Lorestan University, Tehran, Iran , Shams.n@lu.ac.ir
3- Department of Microbiology and Food hygiene, Faculty of Veterinary Medicine, Lorestan University, Tehran, Iran
4- Department of Basic Veterinary Science, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

English Extended Abstract: (267 Views)

Aims: Salmonella typhi is a gram-negative pathogen that causes typhoid fever. Since the least expensive way to combat this pathogen is vaccination, access to an effective vaccine is very important.
Materials and Methods: For this purpose, the proteome of this bacterium with 4322 proteins was extracted from the NCBI server and screened for antigenicity and allergenicity. Finally, the remaining proteins were used to predict T-cell-specific epitopes, and two immunogenic recombinant constructs consisting of epitopes and molecular adjuvant were designed (the first construct contains one repeat of each epitope and the second construct contains two repeats of each epitope). Physicochemical properties, secondary and tertiary structures, antigenicity, solubility, immune system stimulation ability, and molecular docking of each construct were evaluated by reliable online servers.
Findings: At the end of the evaluation of 4322 proteins, seven proteins remained, and the process of predicting their MHCI and MHCII epitopes was completed, after re-evaluation of the epitopes, the desired structures were designed. Despite the observed expected results in both immunogenic structures, the second structure showed more stability and antigenic (the antigenicity of the first and second structures was 0.6701 and 0.6760, respectively). While both structures were hydrophilic, the distribution of extended sheet and random coil structures in the second structure was higher than in the first structure. Furthermore, the second structure was able to bind to its cell surface receptor with a lower docking energy and shorter average hydrogen bond length. Both structures were able to direct the immune system towards stimulating cellular immunity, i.e. increasing the secretion of T helper and T cytotoxic cells.
Conclusion: Based on the presented results, it seems that the second construct, which includes two repeats of each epitope, is a more suitable candidate for the development of an immune vaccine against typhoid fever.

Article number: e11

Keywords: Proteome, Epitope, T cell, Recombinant Vaccine, Salmonella, Cellular Immunity, Bioinformatics

Full-Text [PDF 1484 kb] (82 Downloads)

Article Type: Original Research | Subject: Police Medicine Related Technologies
Received: 2024/07/1 | Accepted: 2024/07/13 | Published: 2024/07/17

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