Background: The aim of the present study was to consider the relationship between Shift Work (SW) and Job Satisfaction (JS) among military healthcare personnel. 

 Materials and Methods: This cross-sectional study was conducted on 301 workers from one of the military subspeciality hospitals in 2010, who were selected with stratified random sampling method. The tools used ifor data collection were standardize JSS job satisfaction questioner with 7 subscale included: “Pay and Benefit”, “Supervision”, “Nature of Work”, “Communication”, “Promotion”, “Operating Procedures”, “Coworker” and also a questioner including demographical variable. In this study we used descriptive statistics (mean, percent) and inferential statistics (ANOVA, Duncan Post hoc test) and for analysis of data we used SPSS programmer version 16.  

 Results: Our sample consist of 301 subjects including 122 (40.5%) day Shift Workers (SWO), 65(21.6%) morning and afternoon SWO, 43 (14.3%) routine SWO and 71(23.6%) night SWO. Among all subscales of JS just JS (P=0.021) and two subscale “Pay and Benefit” (P=0.005) and “Operating Procedures” (P=0.009) show significant relationship with SW while others subscale did not. Day worker and morning and afternoon Shift Workers showed the highest JS rather than routine and night ones. 

 Conclusion: Because night work and rotation work are known as the most important factor in dissatisfaction, modify these two factors should be considered in official guidelines.