Background: The aim of this study was to investigate allele frequency of 15 autosomal STR (short tandem repeat) loci for 50 unrelated healthy individual from Kurdish ethnic population of Kermanshah province in Iran. Materials and Methods: Genetic profile prepared utilizing ABI AmpFl STR IdentifilerTM kit. These 15 STR loci included D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA. Results: There were no deviations from Hardy–Weinberg equilibrium except for two loci of D7s820 and D19s433. Based upon the allelic frequencies, several important forensic parameters were calculated including: power of discrimination (PD), polymorphic information content (PIC), power of exclusion (PE) and matching probability (MP). Conclusion: This study confirmed that investigation of these loci for paternity population studies and handling of criminal cases could be used. We compared the allele frequency spectrum detected in this Kurdish population from Kermanshah with allele frequencies from 8 other datasets on three populations with Iranian origin, ie. Iranians in USA, Iranians in Dubai and Fars ethnic group and 5 neighboring populations from AzerbyjanRepublic, Pakistan, Iraqi-Kurdistan, Iraq and Turkey. It was concluded that the population of present study had least similarity with Azerbyjani(12 loci) and most similarity with Turkish(15 loci) populations.