نشریه علمی پژوهشی طب انتظامی Journal of Police Medicine
Background: The aim of this study was to investigate allele frequency of 15 autosomal STR (short tandem repeat) loci for 50 unrelated healthy individual from Kurdish ethnic population of Kermanshah province in Iran. Materials and Methods: Genetic profile prepared utilizing ABI AmpFl STR IdentifilerTM kit. These 15 STR loci included D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA. Results: There were no deviations from Hardy–Weinberg equilibrium except for two loci of D7s820 and D19s433. Based upon the allelic frequencies, several important forensic parameters were calculated including: power of discrimination (PD), polymorphic information content (PIC), power of exclusion (PE) and matching probability (MP). Conclusion: This study confirmed that investigation of these loci for paternity population studies and handling of criminal cases could be used. We compared the allele frequency spectrum detected in this Kurdish population from Kermanshah with allele frequencies from 8 other datasets on three populations with Iranian origin, ie. Iranians in USA, Iranians in Dubai and Fars ethnic group and 5 neighboring populations from AzerbyjanRepublic, Pakistan, Iraqi-Kurdistan, Iraq and Turkey. It was concluded that the population of present study had least similarity with Azerbyjani(12 loci) and most similarity with Turkish(15 loci) populations.
Background: Amphetamine-type stimulants (ATS), such as methamphetamine and ecstasy (MDMA), are a group of substances, mostly synthetic in origin, that generally stimulate the central nervous system (CNS). There has been a growing tendency among Iranian youth for the abuse of methamphetamine and, to a much lesser extent, ecstasy during the past decade. GC-MS methods used to identify and quantify ATS are lengthy, expensive processes, but often rapid analysis is requested. In this study we used colour tests as the simplest and quickest chemical test to identify MDMA and methamphetamine in the forty samples seized in Iran
Materials and Methods: TIn this study 20 samples of ecstasy tablets and 20 samples of crystalline methamphetamine obtained from antinarcotics police of Iran were analyzed. Color tests (Marquis test, Simon’s test, Chen’s test and Gallic acid test) were carried out on the samples. GC-MS method was used to confirm the color of test results.
Results: All tablets containing MDMA gave a dark purple color to Marquis test and dark blue to Simon’s. No color was developed in reaction with Chen’s reagent. For all tablets containing MDMA a dark green color was developed in response to the addition of Gallic acid reagent. All samples containing methamphetamine gave a orange color to Marquis test and deep blue to Simon’s. No color was developed in reaction with Chen’s and Gallic acid reagents. Color test results were confirmed by GC-MS. The range of MDMA content in tablets was 10–60% and the range of methamphetamine content in samples was 30–70%.
Conclusion: The Marquis test allows the distinction between amphetamine and its ring-substituted analogues. Simon’s test is generally used as a test for secondary amines, such as methamphetamine and secondary ring-substituted amphetamines, including MDMA. Chen’s test is used to distinguish ephedrine, pseudoephedrine, norephedrine, phenylpropanolamine and methcathinone from amphetamine and methamphetamine, which do not react with Chen’s test reagent. A fourth test, the gallic acid test, provides a simple means for the distinction of MDMA from amphetamine or methamphetamine. The results showed that color tests can be used in many laboratories which are not equipped with systems analysis.
Background: One of the most important doctors’ problem is related to dissatisfaction and complaints of patients to jurisdiction and its results on their private and career that unfortunately has been increased significantly, despite of science and technology improvements recently. Any how the most important factors in the conviction of the General Surgeons are: Increasing patient population, increasing of Doctors and their Surgeries, involvement of the General Surgeons in Plastic surgery like cosmetic surgery as well as increasing the compensation rate and community awareness.
Materials and Methods: Complaint records of General Surgeons during April 2014 till end of 2013 (20 months) which raised in the Tehran Legal Medicine Committee have been investigated and all related information have been filled in Check List and afterwards analyzed.
Results: Since April 2012 until end of 2013, “1902 complaints” have been recorded which in 205 cases (10.77%) of those, the General Surgeons have been involved.137 cases (66.8%) out of 205 cases malpractice have been recorded that 88 cases (42.9%) were related to cosmetic surgery and 117 cases (57.1%) were related to other surgeries. Highest level of malpractice based on type as following: lack of skills, imprudence and incaution.91 out of 205 cases (44.4%) have been faculty members and 114 persons (55.6%) have not been. 119 cases (58%) of complaints have been related to private centers and 75 cases (36.6%) non- academic governmental centers and 11 cases (5.4%) academic centers.161 persons (78.5%) have been male surgeons and44 persons (21.5%) female surgeons.
Conclusion: No interference in the other specialty and subspecialty fields by general surgeons such as ENT and plastic surgery, scientific and technical skills improvements, proper justification of patients before surgery about possible complications and possibility of incomplete cure as well as relationship improvement between surgeon and patient could impact to reduce of complaints and no conviction of general surgeons.
Abstract
Introduction: Hair analysis versus other biological matrices (blood and urine) has advantages such as sample stability, long-term detection after sampling, and ease of sampling. This analysis includes the preparing steps of washing the contaminants from the hair, extracting the desired material from that and preparing the extracted solution to entering the analyzer. The purpose of this paper was a review on analytical methods of drugs in hair and their applications in forensics Sciences.
Conclusion: The recent rapid development of technology has provided highly sensitive and selective determination methods such as GC-MS/MS and LC-MS/MS. Instead of blood samples, hair might be used for diagnosis of drug treatment in the near future. For this purpose, simpler and more sensitive methods are required.
Introduction: Four types of liabilities of physicians can be noted regarding the existing rules; moral, civil, criminal, and disciplinary. Regarding the lawsuits against physicians, most of the complaints in Iran have a civil and financial aspect, and criminal or disciplinary prosecution of physician is less considered. The aim of this review was to examine the basis and types of liabilities of physicians in accordance with the Islamic Penal Code.
Conclusion: In cases of medical misconduct, the criminal response is announced through the appointment of blood money or Arsh, and although it is legally of different kinds, in these cases, it has only a civil aspect. Relying on such a theory, one can consider the issue without referring to the judiciary and ignoring lawsuits in the judicial authorities. Criminal, legislative, or judicial and executive policy may or may not be consistent. Investigating the nature of blood money, the existing regulations, and medical insurance regulations can help us provide useful strategies that will benefit the medical community and the judiciary as well as people and insurance.
Abstract
Introduction: Extraction of DNA from forensic samples is the first and one of the most crucial steps of any genetic forensic test. Hence, obtaining a suitable protocol of extraction DNA that is easy to extract, cost, time, and quality which is essential in the forensic genetic research.
Aims: The present study was carried out to achieve a suitable, faster and low cost method of DNA extraction from different sample for further molecular study.
Materials & Methods: After preparation of samples, three different DNA extraction methods, including chelex, non-enzymatic and modified rapid alkaline lysis were evaluated. In order to compare the above mentioned methods for evaluation ease of extraction, low cost and time , the quality and quantity of DNA obtained from each method considering OD, DNA concentration and PCR efficiency were investigated.
Results: The findings of this study show that despite the availability and lower cost and time of manual methods, these methods can be substitute for kit method.
Aims: A DNA mixture is an example of at least two individuals having the genetic material mixed together. In criminal cases related to rape or murder, several individuals have been mixed together in crime scenes through sperm, blood, and etc. Identifying the number of people in a mixed DNA sample is very effective in solving criminal cases. The purpose of this study was to investigate the limitations of identity kits for the identification of more than one person's mixed blood samples.
Materials & Methods: This pilot study was conducted at a medical genetic laboratory in Tehran, Iran in 2018-19. The 17 blood mixtures of relatives and non-relatives were combined and then amplified using an identity recognition kit containing 17 STR markers. The number of alleles in each locus was read by XL 3130 Genetic Analyzer to calculate the genotype of the samples and the number of alleles at each specific site and the number of individuals from each blood mix. Samples were analyzed using Gene Mapper 4.1 software.
Findings: Maximum four, six, six and four alleles were observed in the samples of a mixture of two, three and four unrelated and three unrelated individuals, respectively. As a result, it was not possible to accurately detect the number of individuals involved in the samples of more than three individuals using kits with a maximum of 17 markers.
Conclusion: One of the major limitations of conventional STR kits with up to 17 STR markers is the identification of more than three individuals in mixed samples. This limitation is much greater for mixed samples in which individuals are related.
Aims: Suspected blood stains are among common forms of trace evidence found at crime scenes. Therefore, discriminating between human and animal origin for blood stains is one of the fundamental analyses in forensic laboratories. Currently, tests for determining the origin of bloodstains, such as serology and presumptive tests, are time-consuming and destructive to the sample. However, different polymerase chain reaction (PCR) methods can be used as highly sensitive and specific techniques for determining a bloodstain’s origin.
Materials & Methods: This experimental study was performed to evaluate conventional, nested, and multiplex PCR for discriminating between human and animal bloodstains, and using them in criminal investigations. These methods were designed based on the PCR amplification of cytochrome b and 16S ribosomal mitochondrial DNA fragment.
Findings: The presence of a single band 157 and 170 base pair (bp) in nested and conventional PCR, respectively, as well as two bands 157 and 359 in multiplex PCR, indicates a human blood sample. However, the presence of a single band 359 bp in multiplex PCR and multiple bands in conventional PCR points to the sample’s non-human origin.
Conclusion: This study demonstrated that a multiplex PCR assay designed based on the identification of mitochondrial ribosomal 16S gene has high accuracy in differentiating between human and non-human bloodstains, in addition to being affordable in terms of time and cost. This assay can be useful for forensic purposes because the 16S ribosomal mitochondrial fragment is a small human-specific fragment that is easily amplifiable, even in degraded DNA samples from biological materials.
AIMS: The present study investigates the efficiency of biological samples taken from the crime scene on crime detection.
METHODS. This study is a descriptive, documentary and applied. The statistical population of this research includes 60 employees of Zanjan State Criminal Police, including crime scene investigators, lab experts of forensic chemistry and biology, forensic detectives, medical examiners and examining magistrates and prosecuting attorneys of Zanjan province’s Judicature with at least 5 years of executive experience in crime scene investigation. The questionnaire is used as a tool in this study. The validity of the questionnaire was confirmed by the experts and the reliability of the questionnaire, calculated by the Cronbach's alpha coefficient, was 0.939. For analyzing, descriptive and inferential statistics Kolmogorov-Smirnov, Friedman and one sample t-tests were used. The Kolmogorov-Smirnov test showed normal distribution of the data; so the parametric tests were used to examine the variables.
FINDINGS. The results of one sample t-test showed that biological samples of blood, saliva, semen, and hair strand make a significant contribution to the scientific detection of crime. Friedman test for ranking the importance of various factors related to different samples on crime detection rate from the view of the respondents, showed that using modern equipments and technologies, sample quality and officer scientific and technical knowledge have the most priorities.
CONCLUSION. According to the results the best biologic test, from the view of the respondents, in crime detection is DNA typing.
Question | very much | much | medium | Low | very low |
How familiar are you with the new biometric method? |
How much can new biometric methods help police detectives in crime detection? | |||||
What is the amount of training provided by the organization to identify new methods? | |||||
How much does the lack of continuous training in this field affect the performance of intelligence police detectives in crime detection? | |||||
Do you agree with the feeling of not needing necessary training in biometrics? | |||||
How much is the lack of interest in learning new biometric methods? | |||||
What is the amount of training the organization provides to identify new methods? | |||||
How much progress has been made in training human resources in the application of new biometric methods in the country? | |||||
Is the lack of proper budget allocation for providing education effective in providing new biometric methods? | |||||
Does the authorities' lack of knowledge about the applications of the new biometric method in crime detection, as a result of the lack of appropriate budget allocation, affect the use of this method among police detectives? | |||||
Does not allocating appropriate funds for purchasing and setting up new equipment and facilities harm the detection of organized crimes? | |||||
Does the authorities' lack of feeling the need to allocate funds for equipping and launching new biometric tools harm the detection of organized crimes? | |||||
What is the existence of sanctions and their impact on buying and equipping a biometric laboratory? | |||||
How much is the provision of organizational resources in the use of new biometric methods? | |||||
How effective is the absence of experts in the field of new biometric methods? | |||||
How prepared are the human resources of the organization to use the new biometric methods correctly? | |||||
Is the absence of feeling the need to train specialists in the field of new biometric methods evident? | |||||
How successful do you think the use of new biometric methods will be in Police Headquarters? | |||||
How much has been achieved in the provision expert human resources in the use of new biometric methods in the organization? | |||||
Does the frequent transfer of specialists affect the lack of motivation of these people? | |||||
Does the lack of proper motivation of people in using new biometric methods affect the learning of these methods? | |||||
Does not employing experts in the field of new biometric methods cause the failure of using this method? | |||||
Does the absence of a long-term strategic plan in the field of new biometric methods harm the use of this method? |
Variable | Cronbach's alpha coefficients | composite reliability | Maximum squared common variance | The square of the common variance |
Challenges of providing organizational financial resources | 0.717 | 0.887 | 0.522 | 0.271 |
The challenges of providing expert human resources | 0.791 | 0.825 | 0.302 | 0.298 |
Challenges of training human resources | 0.755 | 0.843 | 0.246 | 0.192 |
Using biometric evidence | 0.951 | 0.779 | 0.286 | 0.204 |
Variable | AVE | Challenges of providing organizational financial resources | The challenges of providing expert human resources |
Challenges of training human resources |
Using biometric evidence | |
Challenges of providing organizational financial resources | 0.663 | 0.814 | 0.814 | - | - | - |
The challenges of providing expert human resources |
0.591 | 0.768 | 0.683 | 0.701 | - | - |
Challenges of training human resources |
0.584 | 0.764 | 0.706 | 0.624 | 0.764 | - |
Using biometric evidence | 0.535 | 0.731 | 0.683 | 0.565 | 0.668 | 0.796 |
Variable | Mean | standard deviation | t | Confidence Interval |
Significance level | average interval | |
lower limit | upper limit | ||||||
Challenges of providing organizational financial resources | 3.616 | 0.718 | 8.270 | 0.468 | 0.764 | p<0.001 | 2.532-3.764 |
The challenges of providing expert human resources | 3.272 | 0.507 | 5.175 | 0.167 | 0.377 | p<0.001 | 2.833-3.377 |
Challenges of training human resources | 3.437 | 0.606 | 6.967 | 0.313 | 0.562 | p<0.001 | 2.687-3.526 |
Using biometric evidence | 3.505 | 0.593 | 8.216 | 0.383 | 0.627 | p<0.001 | 2.617-3.627 |
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