fa ارزیابی قدرت تهاجمی سویه ضعیف شده شیگلا دیسانتری تیپ 1به عنوان کاندید تولید واکسن فناوری‌های مرتبط با طب انتظامی Police Medicine Related Technologies مروری سيستماتيک Systematic Review <p style="text-align:justify">&nbsp;</p> <p dir="RTL" style="text-align:justify">ﻫﺪف: ﺷﻨﺎﺳﺎیﯽ ﻧﺴﺨﻪ ﭘﻼزﻣﯿﺪی ژن <em><span dir="LTR">ipaD</span></em> در ﺑﺎﮐﺘﺮی ﺷﯿﮕﻼ دیﺴﺎﻧﺘﺮی ﺗﯿﭗ <span dir="LTR">1</span> و ارزیﺎﺑﯽ ﻗـﺪرت ﺗﻬـﺎﺟﻤﯽ ﺳـﻮیﻪ زﻧـﺪه ﺿـﻌﯿﻒ&nbsp;ﺷﺪه ﺣﺎﺻﻞ از ﺣﺬف ﻧﺴﺨﻪ ﭘﻼﺳﻤﯿﺪی ژن <em><span dir="LTR">ipaD</span></em> در ﺧﻮﮐﭽﻪ ﻫﻨﺪی ﻫﺪف ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ اﺳﺖ.&nbsp;</p> <p dir="RTL" style="margin-left:86pt; text-align:justify">ﻣﻮاد و روشﻫﺎ: ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮنﻫﺎی ﺑﯿﻮﺷﯿﻤﯿﺎیﯽ، آﻧﺘﯽﺑﺎدی و واﮐﻨﺶ زﻧﺠﯿﺮهای ﭘﻠﯿﻤﺮاز، ﮔﻮﻧﻪ و ﺳﺮوﺗﯿﭗ ﺷﯿﮕﻼی ﺟﺪا ﺷـﺪه&nbsp;از ﺑﯿﻤﺎر ﻣﺸﺨﺺ و ﻧﺴﺨﻪ ﭘﻼزﻣﯿﺪی ژن <span dir="LTR">ipa<em>D</em></span> ﺷﻨﺎﺳـﺎیﯽ ﺷـﺪ. ﺳـﻮیﻪ ﺷـﯿﮕﻼی ﺗﺨﻔﯿـﻒ ﺣـﺪت یﺎﻓﺘـﻪ از ﻃﺮیـﻖ ﺣـﺬف ﻧـﺴﺨﻪ&nbsp;ﭘﻼﺳﻤﯿﺪی ژن <em><span dir="LTR">ipaD</span></em> ﺗﻮﻟﯿﺪ و از ﻃﺮیﻖ آزﻣﻮنﻫﺎی اﺷﺎره ﺷﺪه ﺗﺄیﯿﺪ ﺷﺪ. ﻋﺪم ﺣﻀﻮر ژن <em><span dir="LTR">ipaD</span></em> ﺑﻪوﺳﯿﻠﻪ واﮐﻨﺶ زﻧﺠﯿﺮهای ﭘﻠﯿﻤـﺮاز&nbsp;در ﻣﻘﺎیﺴﻪ ﺑﺎ ﺳﻮیﻪ وﺣﺸﯽ ﻣﻮرد آزﻣﺎیﺶ ﻗﺮار ﮔﺮﻓﺖ. رﻗﺖﻫﺎی ﻣﺨﺘﻠﻒ از ﺑﺎﮐﺘﺮی ﮐﺸﺖ ﺷﺪه ایﺠﺎد و ﺗﻌﺪاد ﮐﻠﻮﻧﯽﻫﺎ ﺑﺮای ﻫﺮﮐـﺪامﺷﻤﺎرش ﺷﺪ. رﻗﺖ ﺧﺎﺻﯽ از ﮐﺸﺖ ﮐﻪ ﺑﺮای آزﻣﻮن ورم ﻣﻠﺘﺤﻤﻪ ﭼﺸﻢ ﻣﻨﺎﺳﺐ اﺳﺖ ﺗﻮﻟﯿﺪ ﺷﺪ. از <span dir="LTR">6</span> ﺧﻮﮐﭽﻪ ﻫﻨـﺪی اﺳـﺘﻔﺎده ﺷـﺪ&nbsp;ﮐﻪ ﺑﻪ دوﮔﺮوه ﮐﻨﺘﺮل و آزﻣﺎیﺶ ﺗﻘﺴﯿﻢﺑﻨﺪی ﺷﺪﻧﺪ. ارزیﺎﺑﯽ ﺗﻮاﻧﺎیﯽ ﺗﻬﺎﺟﻤﯽ ﺳﻮیﻪ ﺗﻮﻟﯿﺪ ﺷﺪه ﺑﻪوﺳﯿﻠﻪ آزﻣﺎیﺶ ایﺠﺎد ورم ﻣﻠﺘﺤﻤـﻪ&nbsp;ﺑﺮوی ﺧﻮﮐﭽﻪ ﻫﻨﺪی ﺑﺮرﺳﯽ ﺷﺪ.&nbsp;</p> <p dir="RTL" style="margin-left:85pt; text-align:justify">یﺎﻓﺘﻪﻫﺎ: ﺳﻮیﻪ ﺷﯿﮕﻼ دیﺴﺎﻧﺘﺮی ﺗﯿﭗ<span dir="LTR">1</span> ﺣﺎﺻﻞ ﺑﻪوﺳﯿﻠﻪ آزﻣﺎیﺶ ﺳﺮوﻟﻮژیﮑﯽ و <span dir="LTR">PCR</span> ﺗﺄیﯿﺪ ﺷﺪ. ﻋـﺪم ﺣـﻀﻮر ژن <em><span dir="LTR">ipaD</span></em> در ﺳـﻮیﻪ&nbsp;یﺎد ﺷﺪه ﻧﯿﺰ ﺑﻪ ﺗﺄیﯿﺪ رﺳﯿﺪ. ﻧﺘﺎیﺞ آزﻣﺎیﺶ ایﺠﺎد ورم ﻣﻠﺘﺤﻤﻪ ﻧﺸﺎن داد ﮐﻪ ایﻦ ﺳﻮیﻪ ﻗـﺪرت ﺗﻬـﺎﺟﻢ ﺑـﻪ ﺳـﻠﻮلﻫـﺎی ﻣﯿﺰﺑـﺎن را از&nbsp;دﺳﺖ داده اﺳﺖ.&nbsp;</p> <p dir="RTL" style="margin-left:86pt; text-align:justify">ﻧﺘﯿﺠﻪﮔﯿﺮی: ﻧﺴﺨﻪ ﭘﻼﺳﻤﯿﺪی ژن<em><span dir="LTR">ipaD</span></em>&nbsp; ﻧﻘﺶ ﻣﻬﻤﯽ در ﺗﻬﺎﺟﻢ ﺑﺎﮐﺘﺮی ﺷﯿﮕﻼ دارد و ﺣﺬف ژن ﻣﺬﮐﻮر ﻗﺪرت ﺗﻬﺎﺟﻤﯽ ﺑـﺎﮐﺘﺮی&nbsp;را ﮐﺎﻫﺶ ﻣﯽدﻫﺪ. ﺷﯿﮕﻼ دیﺴﺎﻧﺘﺮی ﺗﺨﻔﯿﻒ ﺣﺪت یﺎﻓﺘﻪ زﻧﺪه ﮐﻪ ژن <em><span dir="LTR">ipaD</span></em> آن ﺣﺬف ﺷﺪه ﺑﺎﺷﺪ، ﻣﯽﺗﻮاﻧﺪ ﮐﺎﻧﺪیـﺪ ﻣﻨﺎﺳـﺒﯽ ﺑـﺮای&nbsp;ﺗﻮﻟﯿﺪ واﮐﺴﻦ ﻋﻠﯿﻪ ﺷﯿﮕﻠﻮزیﺲ ﺑﺎﺷﺪ.</p> <p style="margin-left:85.0pt"><strong>Aim: </strong>This study aims to detect the <em>ipaD</em> plasmid in <em>Shigella dysenteriae</em> type 1 and evaluate the invasive ability of a live, attenuated <em>Shigella dysenteriae type 1</em> generated from deletion of the <em>ipaD</em> plasmid. The attenuated strain was tested in guinea pigs.</p> <p>&nbsp;</p> <p style="margin-left:85.0pt"><strong>Materials and Methods: </strong>We performed biochemical and antibody tests in addition to PCR analysis, to identify the strain and serotype of an isolated <em>Shigella</em> strain. The plasmid version of the <em>ipaD</em> gene was recognized. We generated a live, attenuated <em>Shigella </em>serotype by deletion of the plasmid version of the<em> ipaD </em>gene, which was confirmed by the above mentioned tests. Absence of the <em>ipaD</em> gene was assessed in a PCR reaction and compared to the wild type strain. Various dilutions of cultured bacteria were prepared and colony numbers counted. We prepared specific dilution which was appropriate for conducting the keratoconjunctive test. We used 6 guinea pigs that were divided into two groups, control (n=3) and experimental (n=3). C Invasion capability of produced strain was evaluated by keratoconjunctive test in guinea pigs.</p> <p>&nbsp;</p> <p style="margin-left:85.0pt"><strong>Results:</strong>The produced live attenuated <em>Shigella dysenteriae</em> type 1 was confirmed through serological tests and PCR. Absence of <em>ipaD</em> was confirmed. Results of keratoconjunctive test has shown that this strain lost its ability to invade host cells.</p> <p>&nbsp;</p> <p style="margin-left:85.0pt"><strong>Conclusion: </strong>The plasmid version of <em>ipaD</em> gene plays a critical role in <em>Shigella</em> invasiveness. Deletion of mentioned gene reduces the invasion ability of these bacteria. Therefore, Live attenuated <em>ipaD shigella dysenteriae</em> can be an appropriate candidate to produce vaccine against shigellosis.</p> شیگلا دیسانتریتیپ 1, تخفیف حدت یافته, ورم ملتحمه چشم, خوکچه هندی, کاندید واکسن Shigella dysenteriae type 1, Live attenuated, Keratoconjunctive, Guinea pig, Vaccine candidate 31 39 http://jpmed.ir/browse.php?a_code=A-10-1-9&slc_lang=fa&sid=1 AR Salehi Chaleshtori احمدرضا صالحی چالشتری 10031947532846009320 10031947532846009320 Yes The Department of Biology, the College of Science, the University of Tehran, Tehran, Iran, Iran ﮔﺮوه زیﺴﺖ ﺷﻨﺎﺳﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎیﻪ، داﻧﺸﮕﺎه ﺟﺎﻣﻊ اﻣﺎم ﺣﺴﯿﻦ(ع)، ﺗﻬﺮان، ایﺮان M Saadati مجتبی سعادتی 10031947532846009321 10031947532846009321 No The Department of Biology, the College of Science, the University of Tehran, Tehran, Iran, Iran ﮔﺮوه زیﺴﺖ ﺷﻨﺎﺳﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎیﻪ، داﻧﺸﮕﺎه ﺟﺎﻣﻊ اﻣﺎم ﺣﺴﯿﻦ(ع)، ﺗﻬﺮان، ایﺮان Y Ahsani Arani یاسر احسنی آرانی 10031947532846009322 10031947532846009322 No The Department of Biology, the College of Science, the University of Tehran, Tehran, Iran, Iran ﮔﺮوه زیﺴﺖ ﺷﻨﺎﺳﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎیﻪ، داﻧﺸﮕﺎه ﺟﺎﻣﻊ اﻣﺎم ﺣﺴﯿﻦ(ع)، ﺗﻬﺮان، ایﺮان A Bahador عباس عباس بهادر 10031947532846009323 10031947532846009323 No Microbiology Department, College of Medicine, University of Tehran Medical Sciences, Iran ﮔﺮوه ﻣﯿﮑﺮوﺑﯿﻮﻟﻮژی ﭘﺰﺷﮑﯽ، داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان، ایﺮان M Hosseini مصطفی حسینی 10031947532846009324 10031947532846009324 No The Department of Biology, the College of Science, the University of Tehran, Tehran, Iran, Iran ﮔﺮوه زیﺴﺖ ﺷﻨﺎﺳﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎیﻪ، داﻧﺸﮕﺎه ﺟﺎﻣﻊ اﻣﺎم ﺣﺴﯿﻦ(ع)، ﺗﻬﺮان، ایﺮان M Hesaraki مهدی حصارکی 10031947532846009325 10031947532846009325 No The Department of Biology, the College of Science, the University of Tehran, Tehran, Iran, Iran ﮔﺮوه زیﺴﺖ ﺷﻨﺎﺳﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎیﻪ، داﻧﺸﮕﺎه ﺟﺎﻣﻊ اﻣﺎم ﺣﺴﯿﻦ(ع)، ﺗﻬﺮان، ایﺮان M Doroudian محمد درودیان 10031947532846009326 10031947532846009326 No islamic azad university , youth researchers club , central tehran branch , iran داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ، ﺑﺎﺷﮕﺎه ﭘﮋوﻫﺸﮕﺮان ﺟﻮان، واﺣﺪ ﺗﻬﺮان ﻣﺮﮐﺰی، ایﺮان