Journal of Police Medicine
نشریه علمی پژوهشی طب انتظامی
J Police Med
Medical Sciences
http://jpmed.ir
1
admin
2228-6241
2383-3483
10.30505
en
jalali
1404
2
1
gregorian
2025
5
1
14
1
online
1
fulltext
fa
تأثیر ترکیبی تمرین تناوبی با شدت بالا و مکمل یاری رسوراترول بر تخریب DNA و لیپید در رت‌های دیابتی
The Combined Effect of High-Intensity Interval Training and Resveratrol Supplementation on DNA and Lipid damage in Diabetic Rats
بهداشت انتظامی
Police Health
پژوهشی اصيل
Original Research
<div style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:IRANsharp;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="FA"><span iransharp_printingsize="">اهداف:</span></span></b> <span lang="FA"><span iransharp_printingsize="">مطالعه حاضر </span></span><span lang="FA"><span iransharp_printingsize="">با هدف بررسی تأثیر ترکیبی هشت هفته </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT</span></span><span lang="FA"><span iransharp_printingsize=""> و مکمل‌یاری رسوراترول بر تخریب </span></span><span dir="LTR"><span iransharp_printingsize="">DNA </span></span><span lang="FA"><span iransharp_printingsize="">(</span></span><span dir="LTR"><span iransharp_printingsize="">8-</span></span><span lang="FA"><span iransharp_printingsize="">هیدروکسی‌دئوکسی‌گوانوزین؛ 8-</span></span><span dir="LTR"><span iransharp_printingsize="">OHdG</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">و پراکسیداسیون لیپیدی (مالون‌دی‌آلدئید؛ </span></span><span dir="LTR"><span iransharp_printingsize="">MDA</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">در موش‌های صحرایی دیابتی نوع ۲ انجام شد.</span></span><span dir="LTR"><span style="font-family:"IRANSharp_PrintingSize",sans-serif"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="FA"><span iransharp_printingsize="">مواد و روش‌ها</span></span></b><span lang="FA"><span iransharp_printingsize="">: چهل سر موش صحرایی نر نژاد ویستار به‌طور تصادفی به چهار گروه (هر گروه ۱۰=</span></span><span dir="LTR"><span iransharp_printingsize="">n) </span></span><span lang="FA"><span iransharp_printingsize="">شامل کنترل دیابتی، دیابتی+تمرین، دیابتی+رسوراترول (۱۰ میلی‌گرم/کیلوگرم/روز) و دیابتی+تمرین+رسوراترول تقسیم شدند. دیابت نوع ۲ با ترکیب رژیم غذایی پرچرب و تزریق استرپتوزوتوسین (۳۵ میلی‌گرم/کیلوگرم) القا گردید. پروتکل </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT</span></span><span lang="FA"><span iransharp_printingsize=""> شامل ۶-۱۲ وهله دویدن ۲ دقیقه‌ای با شدت ۸۵-۹۰ درصد سرعت بیشینه و ۳ دقیقه استراحت فعال، پنج روز در هفته به مدت هشت هفته بود. سطوح سرمی 8-</span></span><span dir="LTR"><span iransharp_printingsize="">OHdG</span></span><span lang="FA"><span iransharp_printingsize=""> و </span></span><span dir="LTR"><span iransharp_printingsize="">MDA</span></span><span lang="FA"><span iransharp_printingsize=""> به‌ترتیب با روش الایزا و اسپکتروفتومتری اندازه‌گیری شدند.</span></span><span dir="LTR"><span style="font-family:"IRANSharp_PrintingSize",sans-serif"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="FA"><span iransharp_printingsize="">یافته‌ها</span></span></b><span lang="FA"><span iransharp_printingsize="">: هر دو مداخله </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT</span></span><span lang="FA"><span iransharp_printingsize=""> و رسوراترول به‌طور معنی‌داری 8-</span></span><span dir="LTR"><span iransharp_printingsize="">OHdG </span></span><span lang="FA"><span iransharp_printingsize="">(به‌ترتیب ۰۰۱/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize=""> و ۰۰۵/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">و </span></span><span dir="LTR"><span iransharp_printingsize="">MDA </span></span><span lang="FA"><span iransharp_printingsize="">(به‌ترتیب ۰۰۵/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize=""> و ۰۰۱/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">را در مقایسه با گروه کنترل دیابتی کاهش دادند. مداخله ترکیبی بیشترین کاهش را در هر دو نشانگر نشان داد و تفاوت معنی‌داری با گروه </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT </span></span><span lang="FA"><span iransharp_printingsize="">(</span></span><span dir="LTR"><span iransharp_printingsize="">8-OHdG</span></span><span lang="FA"><span iransharp_printingsize="">:</span></span> <span lang="FA"><span iransharp_printingsize="">۰۰۰۵/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize=""> ؛۰۰۵/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span> <span dir="LTR"><span iransharp_printingsize="">MDA: </span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">و گروه رسوراترول (8-</span></span><span dir="LTR"><span iransharp_printingsize="">OHdG</span></span><span lang="FA"><span iransharp_printingsize="">:</span></span> <span lang="FA"><span iransharp_printingsize="">۰۰1/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize=""> ؛ </span></span><span dir="LTR"><span iransharp_printingsize="">MDA</span></span><span lang="FA"><span iransharp_printingsize="">:</span></span> <span lang="FA"><span iransharp_printingsize="">۰۰۵/۰></span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span> <span lang="FA"><span iransharp_printingsize="">داشت. بین دو گروه </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT</span></span><span lang="FA"><span iransharp_printingsize=""> و رسوراترول تفاوت معنی‌داری مشاهده نشد (به‌ترتیب ۴۸۷/۰=</span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize=""> و ۴۹۱/۰=</span></span><span dir="LTR"><span iransharp_printingsize="">P</span></span><span lang="FA"><span iransharp_printingsize="">)</span></span><span dir="LTR"><span style="font-family:"IRANSharp_PrintingSize",sans-serif"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="FA"><span iransharp_printingsize="">نتیجه‌گیری</span></span></b><span lang="FA"><span iransharp_printingsize="">: هر دو مداخله </span></span><span dir="LTR"><span iransharp_printingsize="">HIIT</span></span><span lang="FA"><span iransharp_printingsize=""> و رسوراترول به‌طور مستقل باعث کاهش تخریب </span></span><span dir="LTR"><span iransharp_printingsize="">DNA</span></span><span lang="FA"><span iransharp_printingsize=""> و پراکسیداسیون لیپیدی در موش‌های دیابتی می‌شوند. ترکیب این دو مداخله اثر همافزایی نشان داده و محافظت بیشتری در برابر آسیب اکسیداتیو نسبت به هر یک به‌تنهایی ایجاد می‌کند که نشان‌دهنده یک راهبرد غیردارویی مؤثر در مدیریت عوارض دیابت می‌باشد.</span></span><span dir="LTR"><span style="font-family:"IRANSharp_PrintingSize",sans-serif"></span></span></span></span></span><br>
</span></span></div>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span cambria="" style="font-family:">Aims</span></span></b><span style="font-size:11.0pt"><span cambria="" style="font-family:">: This study aimed to investigate the combined effect of eight weeks of HIIT and resveratrol supplementation on DNA damage (8-hydroxydeoxyguanosine; 8-OHdG) and lipid peroxidation (malondialdehyde; MDA) in type 2 diabetic rats.</span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span cambria="" style="font-family:">Materials and Methods</span></span></b><span style="font-size:11.0pt"><span cambria="" style="font-family:">: Forty male Wistar rats were randomly divided into four groups: diabetic control, diabetic+HIIT, diabetic+resveratrol (10 mg/kg/day), and diabetic+HIIT+resveratrol. Type 2 diabetes was induced by a high-fat diet combined with streptozotocin injection (35 mg/kg). The HIIT protocol consisted of 6-12 intervals of 2 minutes at 85-90% of maximum speed with 3-minute active recovery, performed five days per week for eight weeks. Serum levels of 8-OHdG and MDA were measured using ELISA and spectrophotometric methods, respectively.</span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span cambria="" style="font-family:">Results</span></span></b><span style="font-size:11.0pt"><span cambria="" style="font-family:">: Both HIIT and resveratrol significantly reduced 8-OHdG (P<0.001 and P<0.005, respectively) and MDA (P<0.005 and P<0.001, respectively) compared to the diabetic control group. The combined intervention demonstrated the most substantial reductions in both markers, with significant differences compared to HIIT alone (8-OHdG: P<0.005; MDA: P<0.005) and resveratrol alone (8-OHdG: P<0.001; MDA: P<0.005). No significant differences were observed between HIIT and resveratrol groups for either marker (P=0.487 and P=0.491, respectively).</span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span cambria="" style="font-family:">Conclusion</span></span></b><span style="font-size:11.0pt"><span cambria="" style="font-family:">: Both HIIT and resveratrol independently reduce DNA damage and lipid peroxidation in diabetic rats. The combination of these interventions exhibits a synergistic effect, resulting in superior protection against oxidative damage compared to either intervention alone, suggesting a promising non-pharmacological strategy for managing diabetic complications.</span></span></span></span><br>
تمرین تناوبی با شدت بالا, رسوراترول, تخریب DNA, پراکسیداسیون لیپیدی, دیابت نوع ۲, استرس اکسیداتیو
High-intensity interval training, Resveratrol, DNA damage, Lipid peroxidation, Type 2 diabetes, Oxidative stress
0
0
http://jpmed.ir/browse.php?a_code=A-10-536-7&slc_lang=fa&sid=1
Hamdollah
Hadi
حمداله
هادی
amir.hadi1@gmail.com